《植物生理学报》 2017, 53(11): 2037-2043

永福报春苣苔离体快繁体系的建立

闫海霞, 邓杰玲, 何荆洲, 黄昌艳, 崔学强, 卜朝阳^*

广西壮族自治区农业科学院花卉研究所, 南宁530007

通信作者：卜朝阳；E-mail: yangnv@126.com

摘　要：

以永福报春苣苔(Primulina yungfuensis)的叶片为外植体, 开展附加不同植物生长调节剂的培养基对不定芽诱导、增殖培养、壮苗培养、生根培养的对比实验, 筛选出各培养阶段适宜的培养基, 建立其离体快繁体系。结果表明: 适宜的不定芽诱导培养基为MS+3.0 mg·L^-1 6-苄氨基腺嘌呤(6-BA)+0.25 mg·L^-1萘乙酸(NAA); 适宜的不定芽增殖培养基为MS+1.0~3.0 mg·L^-1玉米素(ZT)+0.25 mg·L^-1吲哚丁酸(IBA); 适宜的壮苗培养基为1/2MS+0.1 mg·L^-1 IBA+0.1 g·L^-1活性炭; 适宜的生根培养基为1/2MS+0.3 mg·L^-1 IBA+0.5 g·L^-1活性炭, 生根率为100%; 采用腐殖土和粗沙2:1 (V/V)为移栽基质, 移栽成活率达100%。

关键词：苦苣苔; 永福报春苣苔; 离体快繁

收稿：2017-07-10   修定：2017-09-26

资助：广西科学研究与技术开发计划项目(桂科攻1598006-5-8)和广西农科院基本科研业务专项(2015YT89和桂农科2017YZ05)。

Establishment of in vitro rapid propagation system of Primulina yungfuensis

YAN Hai-Xia, DENG Jie-Ling, HE Jing-Zhou, HUANG Chang-Yan, CUI Xue-Qiang, BU Zhao-Yang^*

Flower Research Institute, Guangxi Academy of Agricultural Sciences, Nanning 530007, China

Corresponding author: BU Zhao-Yang; E-mail: yangnv@126.com

Abstract:

The leaves of Primulina yungfuensis were used as explants. Medium compounding proportion of different plant growth regulators were studied on adventitious bud induction, proliferation, strong seedling, and root regeneration. Thereafter the most suitable medium for rapid propagation in vitro was selected, and the rapid propagation in vitro system was established. The results show that: on MS medium containing 3.0 mg·L^-1 6-benzylaminopurine (6-BA) and 0.25 mg·L^-1 1-naphthylacetic acid (NAA), leaves could be optimally induced to adventitious buds; on MS medium containing 1.0–3.0 mg·L^-1 zeatin (ZT) and 0.25 mg·L^-1 3-indolebutyric acid (IBA), adventitious buds could be optimally proliferated; on 1/2MS medium containing 0.1 mg·L^-1 IBA and 0.1 g·L^-1 activated carbon, adventitious buds could become optimally strong seedling; well-grown adventitious buds were inoculated on 1/2MS medium containing 0.3 mg·L^-1 IBA and 0.5 g·L^-1 activated carbon, and 100% rooting rate was obtained. Using transplanting medium with humus soil and coarse sediment (2:1, V/V), the transplanting survival rate was 100%.

Key words: Gesneriaceae; Primulina yungfuensis; in vitro rapid propagation